SEC62 Antibody
| Code | Size | Price |
|---|
| PSI-6471-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6471-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SEC62 Antibody: HTP1, TP-1, Dtrp1, TLOC1, Translocation protein SEC62, Translocation protein 1
Application Note:
SEC62 antibody can be used for detection of SEC62 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SEC62 Antibody: SEC62 is an integral membrane protein located in the rough endoplasmic reticulum (ER) and is part of the SEC61-SEC62-SEC63 complex that is the central component of the protein translocation apparatus of the ER membrane. It is speculated that SEC61-SEC62-SEC63 may perform post-translational protein translocation into the ER and might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Silencing of this gene with RNAi dramatically reduced the migration and invasive potential of numerous tumor cell lines, suggesting that it may be an attractive target for therapy of various tumors.
Background References:
- Deshaies RJ and Scheckman R. SEC62 encodes a putative membrane protein required for protein translocation in the yeast endoplasmic reticulum. J. Cell Biol. 1989; 109:2653-64.
- Meyer HA, Grau H, Kraft R, et al. Mammalian Sec61 is associated with Sec62 and Sec63. J. Biol. Chem. 2000; 275:14550-7
- Greiner M, Kreutzer B, Jung V, et al. Silencing of the SEC62 gene inhibits migratory and invasive potential of various tumor cells. Int. J. Cancer 2011; 128:2284-95.
Buffer:
SEC62 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
SEC62 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SEC62.
The immunogen is located within amino acids 270 - 320 of SEC62.
The immunogen is located within amino acids 270 - 320 of SEC62.
NCBI Gene ID #:
7095
NCBI Official Name:
SEC62 homolog (S. cerevisiae)
NCBI Official Symbol:
SEC62
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003253
Protein GI Number:
4507525
Purification:
SEC62 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist.
Swissprot #:
Q99442
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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