NETO1 Antibody
| Code | Size | Price |
|---|
| PSI-6469-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6469-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NETO1 Antibody: BCTL1, BTCL1, Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1
Application Note:
NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
NETO1 Antibody: Neuropilin and tolloid-like protein 1 (NETO1) is involved in the development and/or maintenance of neuronal circuitry. It is a type I transmembrane protein that is expressed in the brain and retina. NETO1 contains one LDL-receptor class A domain and two CUB domains and is either membrane-bound or secreted. It has three alternatively spliced isoforms, the first two of which are retina-specific and the third of which is found in both retina and brain tissue. Furthermore, as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), it regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.
Background References:
- Michishita M, Ikeda T, Nakashiba T, et al. A novel gene, Btcl1, encoding CUB and LDLa domains is expressed in restricted areas of mouse brain. Biochem. Biophys. Res. Commun. 2003; 306:680-6.
- Stöhr H, Berger C, Fröhlich S, et al. A novel gene encoding a putative transmembrane protein with two extracellular CUB domains and a low-density lipoprotein class A module: isolation of alternatively spliced isoforms in retina and brain. Gene 2002; 286:223-31
- Straub C, Hunt DL, Yamasaki M, et al. Distinct functions of kainate receptors in the brain are determined by the auxiliary subunit Neto1. Nat. Neurosci. 2011; 14:866-73.
- Ng D, Pitcher GM, Szilard RK, et al. Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning. PLoS Biol. 2009; 7:e41
Buffer:
NETO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
NETO1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NETO1.
The immunogen is located within amino acids 350 - 400 of NETO1.
The immunogen is located within amino acids 350 - 400 of NETO1.
NCBI Gene ID #:
81832
NCBI Official Name:
neuropilin (NRP) and tolloid (TLL)-like 1
NCBI Official Symbol:
NETO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 60 kDa
Observed: 60 kDa
Observed: 60 kDa
Protein Accession #:
NP_694821
Protein GI Number:
20452470
Purification:
NETO1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
NETO1 antibody is predicted to not cross-react with other NETO protein family members.
Swissprot #:
Q8TDF5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier |
|---|