DPAGT1 Antibody
| Code | Size | Price |
|---|
| PSI-6485-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6485-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
DPAGT1 Antibody: GPT, ALG7, DGPT, G1PT, UAGT, UGAT, CDG1J, DPAGT, CDG-Ij, CMSTA2, DPAGT2, D11S366, UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, GlcNAc-1-P transferase
Application Note:
DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DPAGT1 Antibody: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.
Background References:
- Wu X, Rush JS, Karaoglu D, et al. Deficiency of UDP-GlcNAc:Dolichol Phosphate N-Acetylglucosamine-1 Phosphate Transferase (DPAGT1) causes a novel congenital disorder of glycosylation type Ij. Hum. Mutat. 2003; 22:144-50.
- Bretthauer RK. Structure, expression, and regulation of UDP-GlcNAc:dolichol phosphate GlcNAc-1-phosphate transferase (DPAGT1). Curr. Drug Targets 2009; 10:477-82
- Sengupta PK, Bouchie MP, and Kukuruzinska MA. N-glycosylation gene DPAGT1 is a target of the Wnt/beta-catenin signaling pathway. J. Biol. Chem. 2010; 285:31164-73.
Buffer:
DPAGT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1.
The immunogen is located within amino acids 30 - 80 of DPAGT1.
The immunogen is located within amino acids 30 - 80 of DPAGT1.
NCBI Gene ID #:
1798
NCBI Official Name:
dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
NCBI Official Symbol:
DPAGT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001373
Protein GI Number:
42794009
Purification:
DPAGT1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least four isoforms of DPAGT1 are known to exist; this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.
Swissprot #:
Q9H3H5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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