EI24 Antibody
| Code | Size | Price |
|---|
| PSI-6487-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6487-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
EI24 Antibody: EPG4, PIG8, TP53I8, p53-induced gene 8 protein
Application Note:
EI24 antibody can be used for detection of EI24 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
EI24 Antibody: The etoposide-induced 24 (EI24) protein is an immediate-early induction target of p53-mediated apoptosis and functions as a tumor suppressor. EI24 contains six transmembrane domains and is localized to the endoplasmic reticulum (ER). It binds to Bcl-2 and may contribute to apoptosis by modulating the activity and function of Bcl-2. EI24 is highly mutated in aggressive breast cancers and its loss in breast cancer tissue correlates with tumor invasiveness. Similar findings in invasive cervical carcinoma suggest that EI24 may be a promising therapeutic target in the treatment of invasive cancer.
Background References:
- Lehar SM, Nacht M, Jacks T, et al. Identification and cloning of EI24, a gene induced by p53 in etoposide-treated cells. Oncogene 1996; 12:1181-7.
- Gu Z, Flemington C, Chittenden T, et al. ei24, a p53 response gene involved in growth suppression and apoptosis. Mol. Cell Biol. 2000; 20:233-41
- Zhao X, Ayer RE, Davis SL, et al. Apoptosis factor EI24/PIG8 is a novel endoplasmic reticulum-localized Bcl-2-binding protein which is associated with suppression of breast cancer invasiveness. Cancer Res. 2005; 65:2125-9.
- Mazumder D, Mitra S, Singh RK, et al. Inactivation of CHEK1 and EI24 are associated with the development of invasive cervical carcinoma: clinical and prognostic implications. Int. J. Cancer 2010; epub
Buffer:
EI24 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
EI24 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human EI24.
The immunogen is located within the last 50 amino acids of EI24.
The immunogen is located within the last 50 amino acids of EI24.
NCBI Gene ID #:
9538
NCBI Official Name:
etoposide induced 2.4 mRNA
NCBI Official Symbol:
EI24
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004870
Protein GI Number:
55956766
Purification:
EI24 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
At least two isoforms of EI24 are known to exist; this antibody will only detect the larger isoform.
Swissprot #:
O14681
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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