SPATA3 Antibody

ProSci
Product Code: PSI-6551
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6551-0.02mg0.02mg£150.00
Quantity:
PSI-6551-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of SPATA3 in 3T3 cell lysate with SPATA3 antibody at 1 μg/mL.
2 / 3
Immunohistochemistry of SPATA3 in mouse testis tissue with SPATA3 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of SPATA3 in mouse testis tissue with SPATA3 antibody at 20 μg/mL.

Western blot analysis of SPATA3 in 3T3 cell lysate with SPATA3 antibody at 1 μg/mL.
Immunohistochemistry of SPATA3 in mouse testis tissue with SPATA3 antibody at 2.5 μg/mL.
Immunofluorescence of SPATA3 in mouse testis tissue with SPATA3 antibody at 20 μg/mL.

Further Information

Additional Names:
SPATA3 Antibody: TSARG1, TSARG1, Spermatogenesis-associated protein 3, Testis and spermatogenesis cell-related protein 1
Application Note:
SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SPATA3 Antibody: The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells.
Background References:
  • Fu JJ, Lu GX, Li LY, et al. Molecular cloning for testis spermatogenesis cell apoptosis related gene TSARG1 and Mtsarg1 and expression analysis for Mtsarg1 gene. Yi Chuan Xue Bao 2003; 30:25-9.
  • Yang HM, Liu G, Nie ZY, et al. Molecular cloning of a novel rat gene Tsarg1, a member of the DnaJ/HSP40 protein family. DNA Seq. 2005; 16:166-72.
Buffer:
SPATA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SPATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPATA3.

The immunogen is located within the last 50 amino acids of SPATA3.
NCBI Gene ID #:
130560
NCBI Official Name:
spermatogenesis associated 3
NCBI Official Symbol:
SPATA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 21 kDa

Observed: 26 kDa
Protein Accession #:
NP_620712
Protein GI Number:
31712020
Purification:
SPATA3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
At least three isoforms of SPATA3 are known to exist; this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members
Swissprot #:
Q8NHX4
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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