SPATA4 Antibody

ProSci
Product Code: PSI-6553
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6553-0.02mg0.02mg£150.00
Quantity:
PSI-6553-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of SPATA4 in mouse testis tissue with SPATA4 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of SPATA4 in mouse testis tissue with SPATA4 antibody at 20 μg/mL.

Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SPATA4 in mouse testis tissue with SPATA4 antibody at 2.5 μg/mL.
Immunofluorescence of SPATA4 in mouse testis tissue with SPATA4 antibody at 20 μg/mL.

Further Information

Additional Names:
SPATA4 Antibody: SPEF1B, TSARG2, Spermatogenesis-associated protein 4, Testis and spermatogenesis cell-related protein 2
Application Note:
SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SPATA4 Antibody: The spermatogenesis associated protein 4 (SPATA4) protein, also known as TSARG1, is highly expressed in testis, and to a much lower extent in other tissues. SPATA4 contains a highly conserved DUF1042 domain, but no significant homology with any other known protein. SPATA4 is thought to play an important role in the development of cryptorchid testis and is a testis-specific apoptosis candidate oncogene.
Background References:
  • Liu SF, Lu GX, Liu G, et al. Cloning of a full-length cDNA of human testis-specific spermatogenic cell apoptosis inhibitor TSARG2 as a candidate oncogene. Biochem. Biophys. Res. Commun. 2004; 319:32-40
  • Liu SF, Ai C, Ge ZQ, et al. Molecular cloning and bioinformatic analysis of SPATA4 gene. J. Biochem. Mol. Biol. 2005; 38:739-47.
Buffer:
SPATA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SPATA4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA4.

The immunogen is located within amino acids 200 - 250 of SPATA4.
NCBI Gene ID #:
132851
NCBI Official Name:
spermatogenesis associated 4
NCBI Official Symbol:
SPATA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_653245
Protein GI Number:
31543825
Purification:
SPATA4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
At least two isoforms of SPATA4 are known to exist; this antibody will detect both isoforms. SPATA4 antibody is predicted to not cross-react with SPATA protein family members
Swissprot #:
Q8NEY3
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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