AFAP1L Antibody
| Code | Size | Price |
|---|
| PSI-6559-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6559-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
AFAP1L Antibody: Actin filament-associated protein 1-like 1, AFAP1-like protein 1
Application Note:
AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
AFAP1L Antibody: The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation (1, 4). Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.
Background References:
- Snyder BN, Cho Y, Qian Y, et al. AFAP1L1 is a novel adaptor protein of the AFAP family that interacts with cortactin and localizes to invadosomes. Eur. J. Cell Biol. 2011; 90:376-89
- Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993;13:7892-900.
- Baisden JM, Gatesman AS, Cherezova L, et al. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene 2001; 20:6607-16.
- Saltel F, Daubon T, Juin A, et al. Invadosomes: intriguing structures with promise. Eur. J. Cell Biol. 2011; 90:100-7.
Buffer:
AFAP1L Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (79%)
Immunogen:
AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1.
The immunogen is located within amino acids 70 - 120 of AFAP1L.
The immunogen is located within amino acids 70 - 120 of AFAP1L.
NCBI Gene ID #:
134265
NCBI Official Name:
actin filament associated protein 1-like 1
NCBI Official Symbol:
AFAP1L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_689619
Protein GI Number:
55742689
Purification:
AFAP1L Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members.
Swissprot #:
Q8TED9
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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