PRICKLE1 Antibody
| Code | Size | Price |
|---|
| PSI-6561-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6561-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PRICKLE1 Antibody: RILP, EPM1B, RILP, Prickle-like protein 1
Application Note:
PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.
Background References:
- Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56
- Shimojo M and Hersh LB. REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor. Mol. Cell. Biol. 2003; 23:9025-31
- Chan DW, Chan CY, Yam JW, et al. Prickle1 negatively regulates Wnt/β-catenin pathway by promoting Disheveled ubiquitination/degradation in liver cancer. Gastroenterology 2006; 131:1218-27.
- Tao H, Manak JR, Sowers L, et al. Mutations in prickle orthologs cause seizures in flies, mice, and humans. Am. J. Hum. Genet. 2011; 88:138-49.
Buffer:
PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1.
The immunogen is located within the last 50 amino acids of PRICKLE1.
The immunogen is located within the last 50 amino acids of PRICKLE1.
NCBI Gene ID #:
144165
NCBI Official Name:
prickle homolog 1
NCBI Official Symbol:
PRICKLE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46, 91 kDa
Observed: 52, 90 kDa
Observed: 52, 90 kDa
Protein Accession #:
NP_694571
Protein GI Number:
222136684
Purification:
PRICKLE1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.
Swissprot #:
Q96MT3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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