PRICKLE2 Antibody
| Code | Size | Price |
|---|
| PSI-6563-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6563-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PRICKLE2 Antibody: EPM5, Prickle-like protein 2
Application Note:
PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
PRICKLE2 Antibody: PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the related protein PRICKLE1 are expressed in postmitotic neurons and promote neurite outgrowth, and both proteins promote neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. PRICKLE2 localizes to the postsynaptic density and interacts with PSD-95 and NMDA receptors. Defects in the gene encoding PRICKLE2 are associated with autosomal recessive progressive myoclonic epilepsy.
Background References:
- Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56.
- Veeman MT, Slusarski DC, Kaykas A, et al. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr. Biol. 2003; 13:680-5.
- Okuda H, Miyata S, Mori Y, et al. Mouse Prickle1 and Prickle2 are expressed in postmitotic neurons and promote neurite outgrowth. FEBS Lett. 2007; 581:4754-60
- Fujimura L, Watanabe-Takano H, Sato Y, et al. Prickle promotes neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. Neurosci. Lett. 2009; 467:6-10.
Buffer:
PRICKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PRICKLE2 antibody was raised against a 15 amino acid synthetic peptide near the center of human PRICKLE2.
The immunogen is located within amino acids 420 - 470 of PRICKLE2.
The immunogen is located within amino acids 420 - 470 of PRICKLE2.
NCBI Gene ID #:
166336
NCBI Official Name:
prickle homolog 2
NCBI Official Symbol:
PRICKLE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_942559
Protein GI Number:
38524620
Purification:
PRICKLE2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members.
Swissprot #:
Q7Z3G6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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