PRICKLE4 Antibody

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ProSci
Product Code: PSI-6567
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6567-0.02mg0.02mg£150.00
Quantity:
PSI-6567-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 μg/ml in the (A) absence and (B) presence of blocking peptide.
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Immunocytochemistry of PRICKLE4 in A549 cells with PRICKLE4 antibody at 5 μg/mL.
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Immunofluorescence of PRICKL4 in A549 cells with PRICKLE4 antibody at 20 μg/mL.
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Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 μg/ml in the (A) absence and (B) presence of blocking peptide.
Immunocytochemistry of PRICKLE4 in A549 cells with PRICKLE4 antibody at 5 μg/mL.
Immunofluorescence of PRICKL4 in A549 cells with PRICKLE4 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
PRICKLE4 Antibody: OBTP, OEBT, TOMM6, C6orf49, Prickle-like protein 4, Overexpressed breast tumor protein
Application Note:
PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PRICKLE4 Antibody: PRICKLE4, also known as over-expressed breast tumor protein (OBTP), is a member of the LIM family of proteins. It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus. Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation. Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.
Background References:
  • Teufel A, Weinmann A, Galle PR, et al. Characterization of OEBT, a LIM protein. Int. J. Mol. Med. 2005; 15:513-8.
  • Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56
  • Veeman MT, Slusarski DC, Kaykas A, et al. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr. Biol. 2003; 13:680-5
Buffer:
PRICKLE4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PRICKLE4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PRICKLE4.

The immunogen is located within the first 50 amino acids of PRICKLE4.
NCBI Gene ID #:
29964
NCBI Official Name:
prickle homolog 4
NCBI Official Symbol:
PRICKLE4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_037529
Protein GI Number:
118722347
Purification:
PRICKLE4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.
Swissprot #:
Q2TBC4
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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