PIWI-L2 Antibody
| Code | Size | Price |
|---|
| PSI-6569-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6569-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PIWI-L2 Antibody: CT80, HILI, mili, PIWIL1L, Piwi-like protein 2, CT80
Application Note:
PIWI-L2 antibody can be used for detection of PIWI-L2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PIWI-L2 Antibody: PIWI-L2 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L2 has been implicated in multiple roles including stem cell self-renewal and is highly expressed in many breast cancers. In these tumors, PIWI-L2 silences the expression of STAT3, a regulator of Bcl-XL and cyclin D1, allowing the proliferation and survival of breast cancer stem cells. Recent studies have shown that overexpression of PIWI-like proteins are potential biomarkers for colon and other cancers.
Background References:
- Kuramochi-Miyagawa S, Kimura T, Yomogida K, et al. Two mouse piwi-related genes: miwi and mili. Mech. Dev. 108:121-33
- Thomson T and Lin H. The biogenesis and function PIWI proteins and piRNAs: progress and prospect. Annu. Rev. Cell Dev. Biol. 2009; 25:355-76.
- Lee JH, Jung C, Javadian-Elyaderani P, et al. Pathways of proliferation and antiapoptosis driven in breast cancer stem cells by stem cell protein Piwil2. Cancer Res. 2010; 70:4569-79.
- Li L, Yu C, Gao H, et al. Argonaute proteins: potential biomarkers for human colon cancer. BMC Cancer 2010; 10:38
Buffer:
PIWI-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (87%), Chicken: (75%)
Immunogen:
PIWI-L2 antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2.
The immunogen is located within amino acids 410 - 460 of PIWI-L2.
The immunogen is located within amino acids 410 - 460 of PIWI-L2.
NCBI Gene ID #:
55124
NCBI Official Name:
piwi-like 2
NCBI Official Symbol:
PIWIL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_00129193
Protein GI Number:
24431985
Purification:
PIWI-L2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.
Swissprot #:
Q8TC59
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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