CD33 Antibody

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ProSci
Product Code: PSI-6595
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6595-0.02mg0.02mg£150.00
Quantity:
PSI-6595-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 μg/mL.
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Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
CD33 Antibody: p67, SIGLEC3, SIGLEC-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3
Application Note:
CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
CD33 Antibody: CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family that is highly expressed on myeloid progenitor cells. Assessment of CD33 expression is of great importance in the immunodiagnosis of acute leukemia, allowing distinction between myeloid and lymphoid origin, as CD33 is generally restricted to the myelomonocytic lineage. CD33 can associate with the protein-tyrosine phosphatases SHP-1 and SHP-2 and thus could modulate downstream signaling events associated with cell activation. Common variants of CD33 have been found to be associated with late-onset Alzheimer's disease.
Background References:
  • Freeman SD, Kelm S, Barber EK, et al. Characterization of CD33 as a new member of the sialoadhesion family of cellular interaction molecules. Blood 1995; 85:2005-12.
  • Crocker PR and Varki A. Siglecs, sialic acids, and innate immunity. Trends Immunol. 2001; 22:337-42.
  • Taylor VC, Buckley CD, Douglass M, et al. The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2. J. Biol. Chem. 1999; 274:11505-12
  • Naj AC, Jun G, Beecham GW, et al. Common variants at MS4A4/MS4A6E, CD2AP, CD33 and EPHA are associated with late-onset Alzheimer's disease. Nat. Genet. 2011; 43:436-41
Buffer:
CD33 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CD33 antibody was raised against a 14 amino acid synthetic peptide near the center of human CD33.

The immunogen is located within amino acids 180 - 230 of CD33.
NCBI Gene ID #:
945
NCBI Official Name:
CD33 molecule
NCBI Official Symbol:
CD33
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001763
Protein GI Number:
130979981
Purification:
CD33 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least three isoforms of CD33 are known to exist; this antibody will detect all three isoforms.
Swissprot #:
P20138
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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