LEMD1 Antibody

ProSci
Product Code: PSI-6599
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6599-0.02mg0.02mg£150.00
Quantity:
PSI-6599-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

Further Information

Additional Names:
LEMD1 Antibody: CT50, LEMP-1, LEM domain-containing protein 1, LEM domain protein 1, CT50
Application Note:
LEMD1 antibody can be used for detection of LEMD1 by Western blot at 1 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
LEMD1 Antibody: LEMD1 (LEM domain-containing1) is a member of cancer-testis gene family. LEMD1 is normally expressed only in testis. Six alternatively spliced forms of LEMD1 transcripts in normal testis have been identified, but only one of the six was expressed in colorectal cancers. LEMD1 may represent a promising target antigen for immunotherapy of colorectal cancers. In addition, LEMD1 and SPATA19 are putative cancer biomarkers and promising targets for active immunotherapy.
Background References:
  • Yuki D, Lin YM, Fujii Y, et al. Isolation of LEM domain-containing 1, a novel testis-specific gene expressed in colorectal cancers. Oncol. Rep. 2004; 12:275-80
  • Ghafouri-Fard S, Ousati Ashtiani Z, Sabah Golian B, et al. Expression of two testis-specific genes, SPATA19 and LEMD1, in prostate cancer. Arch. Med. Res. 2010; 41:195-200
Buffer:
LEMD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LEMD1 antibody was raised against a 19 amino acid synthetic peptide near the center of human LEMD1.

The immunogen is located within amino acids 90 - 140 of LEMD1.
NCBI Gene ID #:
93273
NCBI Official Name:
LEM domain containing 1
NCBI Official Symbol:
LEMD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q68G75
Protein GI Number:
312261248
Purification:
LEMD1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least six isoforms of LEMD1 are known to exist; this antibody will detect the longest isoform. LEMD1 antibody is predicted to not cross-react with LEMD2 and LEMD3.
Swissprot #:
Q68G75
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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