SIVA Antibody

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ProSci
Product Code: PSI-6621
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6621-0.02mg0.02mg£150.00
Quantity:
PSI-6621-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of SIVA in mouse liver tissue lysate with SIVA antibody at 1 μg/mL.
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Immunofluorescence of SIVA in mouse liver cells with SIVA antibody at 20 μg/mL.
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Western blot analysis of SIVA in mouse liver tissue lysate with SIVA antibody at 1 μg/mL.
Immunofluorescence of SIVA in mouse liver cells with SIVA antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
SIVA Antibody: SIVA, CD27BP, Siva-1, Siva-2, SIVA, Apoptosis regulatory protein Siva, CD27-binding protein
Application Note:
SIVA antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SIVA Antibody: SIVA plays an important role in the apoptotic pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. SIVA is a small cysteine-rich protein with a unique N-terminal amphipathic helical region, a death domain homology region (DDHR) and a C-terminal cysteine-rich B-box-like or zinc-finger-like structure. The DDHR domain binds to CD27 and induces apoptosis in T cells through a caspase-dependent mitochondrial pathway. SIVA inhibits BCL2L1 isoform Bcl-xL anti-apoptotic activity and inhibits activation of NFκ-B and promotes T-cell receptor-mediated apoptosis. SIVA represents a potential therapeutic target for the treatment of neuronal cell death diseases.
Background References:
  • Prasad KV, Ao Z, Yoon Y, et al. CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc. Natl. Acad. Sci. USA 1997; 94:6346-51.
  • Py B, Slomianny C, Auberger P, et al. Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway. J. Immunol. 2004; 172:4008-17.
  • Resch U, Schichl YM, Winsauer G, et al. Siva1 is a XIAP-interacting protein that balances NFkappaB and JNK signalling to promote apoptosis. J. Cell Sci. 2009; 122:2651-61
  • Jacobs SB, Basak S, Murray JI, et al. Siva is an apoptosis-selective p53 target gene important for neuronal cell death. Cell Death Differ. 2007; 14:1374-85.
Buffer:
SIVA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (80%)
Immunogen:
SIVA antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human SIVA.

The immunogen is located within the first 50 amino acids of SIVA.
NCBI Gene ID #:
10572
NCBI Official Name:
SIVA1, apoptosis-inducing factor
NCBI Official Symbol:
SIVA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_068355
Protein GI Number:
11277468
Purification:
SIVA Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
O15304
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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