SIPA1 Antibody
| Code | Size | Price |
|---|
| PSI-6643-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6643-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SIPA1 Antibody: SPA1, SPA1, Signal-induced proliferation-associated protein 1, GTPase-activating protein Spa-1, Sipa-1
Application Note:
SIPA1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SIPA1 Antibody: Signal-induced proliferation 1 (SIPA1) is a member of the SIPA1 family of RapGAPs, a family of mitogen-induced GTPase activating proteins. SIPA1 exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. When abnormally or prematurely expressed, SIPA1 may also hamper mitogen-induced cell cycle progression and may increase metastases in human cancers.
Background References:
- Hattori M, Tsukamoto N, Nur-e-Kamal MS, et al. Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression. Mol. Cell. Biol. 1995; 15:551-60.
- Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci. 2009; 100:17-23.
- Kurachi H, Wada Y, Tsukamoto N, et al. Human SPA-1 gene product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2. Segregate expression profiles from a rap1GAP gene product. J. Biol. Chem. 1997; 272:28081-8.
- Ishida D, Kometani K, Yang H, et al. Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell 2003; 4:55-65.
Buffer:
SIPA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (95%)
Immunogen:
SIPA1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human SIPA1.
The immunogen is located within amino acids 40 - 90 of SIPA1.
The immunogen is located within amino acids 40 - 90 of SIPA1.
NCBI Gene ID #:
6494
NCBI Official Name:
signal-induced proliferation-associated 1
NCBI Official Symbol:
SIPA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH10492
Protein GI Number:
206725523
Purification:
SIPA1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least three isoforms of SIPA1 are known to exist; this antibody will recognize all three isoforms. This antibody is specific for SIPA1 and will not recognize the other SIPA1 family of proteins.
Swissprot #:
Q96FS4
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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