IRF2BP2 Antibody

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ProSci
Product Code: PSI-6705
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6705-0.02mg0.02mg£150.00
Quantity:
PSI-6705-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of IRF2BP2 in HeLa cell lysate with IRF2BP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
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Immunocytochemistry of IRF2BP2 in A20 cells with IRF2BP2 antibody at 2.5 μg/mL.
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Western blot analysis of IRF2BP2 in HeLa cell lysate with IRF2BP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunocytochemistry of IRF2BP2 in A20 cells with IRF2BP2 antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
IRF2BP2 Antibody: Interferon regulatory factor 2-binding protein 2, IRF-2-binding protein 2
Application Note:
IRF2BP2 antibody can be used for detection of IRF2BP2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Background:
IRF2BP2 Antibody: IRF2BP2, like the related protein IRF2BP1, is a co-repressor that interacts specifically with the C-terminal repression domain of Interferon Regulatory Factor 2 (IRF2). IRF2BP2 is a direct target gene of p53 and is involved in cell survival during the p53 stress response, able to impede the p53-mediated transactivation of p21 and Bax. IRF2BP2 is also a co-factor of VGLL4 and is required to induce the expression of vascular endothelial growth factor A (VEGF-A) in muscle. It is normally found in the nucleus of skeletal muscle and cardiac cells, but can be found in the cytoplasm during skeletal muscle differentiation.
Background References:
  • Childs KS and Goodbourn S. Identification of novel co-repressor molecules for interferon regulatory factor-2. Nuc. Acids Res. 2003; 31:3016-26
  • Koeppel M, van Heeringen SJ, Smeenk L, et al. The novel p53 target gene IRF2BP2 participates in cell survival during the p53 stress response. Nuc. Acids Res. 2009; 37:322-35.
  • Teng ACT, Kuraitis D, Deeke SA, et al. IRF2BP2 is a skeletal and cardiac muscle-enriched ischemia-inducible activator of VEGFA expression. FASEB J. 2010; 24:4825-34.
  • Teng ACT, Al-montashiri NAM, Cheng BLM, et al. Identification of a phosphorylation-dependent nuclear localization motif in interferon regulatory factor 2 binding protein 2. PLoS One 2011; 6:e24100.
Buffer:
IRF2BP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IRF2BP2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human IRF2BP2.

The immunogen is located within amino acids 440 - 490 of IRF2BP2.
NCBI Gene ID #:
359948
NCBI Official Name:
interferon regulatory factor 2 binding protein 2
NCBI Official Symbol:
IRF2BP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_892017
Protein GI Number:
116734704
Purification:
IRF2BP2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least two isoforms of IRF2BP2 are known to exist; this antibody will detect both. This antibody is predicted to not cross-react with any FOXD4 protein family members.
Swissprot #:
Q7Z5L9
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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