Nephrin Antibody
| Code | Size | Price |
|---|
| PSI-5615-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5615-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Documents
Further Information
Additional Names:
Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor
Application Note:
Nephrin antibody can be used for detection of Nephrin by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
Background References:
- Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein - Nephrin - is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.
- Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5
- Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.
Buffer:
Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%), Rat: (94%)
Immunogen:
Nephrin antibody was raised against a 16 amino acid synthetic peptide from near the center of human Nephrin.
The immunogen is located within amino acids 870 - 920 of Nephrin.
The immunogen is located within amino acids 870 - 920 of Nephrin.
NCBI Gene ID #:
4868
NCBI Official Name:
nephrosis 1, congenital, Finnish type (nephrin)
NCBI Official Symbol:
NPHS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004637
Protein GI Number:
4758822
Purification:
Nephrin Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O60500
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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