B9D1 Antibody

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ProSci
Product Code: PSI-6715
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6715-0.02mg0.02mg£150.00
Quantity:
PSI-6715-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of B9D1 in 293 cell lysate with B9D1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of B9D1 in 293 cells with B9D1 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of B9D1 in 293 cells with B9D1 antibody at 20 μg/mL.
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Western blot analysis of B9D1 in 293 cell lysate with B9D1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of B9D1 in 293 cells with B9D1 antibody at 5 μg/mL.
Immunofluorescence of B9D1 in 293 cells with B9D1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
B9D1 Antibody: B9, MKS9, EPPB9, MKSR1, B9 domain-containing protein 1, MKS1-related protein 1
Application Note:
B9D1 antibody can be used for detection of B9D1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
B9D1 Antibody: Meckel syndrome (MKS) is an embryonic lethal, autosomal recessive disorder characterized by polycystic kidney disease, central nervous system defects, polydactyly and liver fibrosis. B9D1 is a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. B9D1, and its related protein B9D2, form a complex with MKS1, disruption of which causes MKS. B9D1 is thought to be required for normal hedgehog signaling, ciliogenesis, and ciliary protein localization.
Background References:
  • Williams CL, Winkelbauer ME, Schafer JC, et al. Functional redundancy of the B9 proteins and nephocystins in Caenorhabditis elegans ciliogenesis. Mol. Biol. Cell 2008; 19:2154-68.
  • Hopp K, Heyer CM, Hommerding CJ, et al. B9D1 is revealed as a novel Meckel syndrome (MKS) gene by targeted exon-enriched next-generation sequencing and deletion analysis. Hum. Mol. Genet. 2011; 20:2524-34.
  • Dowdle WE, Robinson JF, Kneist A, et al. Disruption of a ciliary B9 protein complex causes Meckel syndrome. Am. J. Hum. Genet. 2011; 89:94-110.
Buffer:
B9D1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
Immunogen:
B9D1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human B9D1.

The immunogen is located within amino acids 130 - 180 of B9D1.
NCBI Gene ID #:
27077
NCBI Official Name:
B9 protein domain 1
NCBI Official Symbol:
B9D1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056496
Protein GI Number:
343478275
Purification:
B9D1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least two isoforms of B9D1 are known to exist; this antibody will only recognize the longest isoform. B9D1 antibody is predicted to not cross-react with other DNAJC family members.
Swissprot #:
Q9UPM9
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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