PHOX2B Antibody

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ProSci
Product Code: PSI-6913
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6913-0.02mg0.02mg£150.00
Quantity:
PSI-6913-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of PHOX2B in 293 cell lysate with PHOX2B antibody at (A) 1 and (B) 2 μg/mL.
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Western blot analysis of PHOX2B in 293 cell lysate with PHOX2B antibody at (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
PHOX2B Antibody: PMX2B, NBLST2, NBPhox, PMX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox
Application Note:
PHOX2B antibody can be used for detection of PHOX2B by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
PHOX2B Antibody: PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired-homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis. PHOX2 proteins are crucial for the regulation of endogenous hydroxylases in neural crest cells and promote sympathetic neuron generation. Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, PHOX2A controls only the differentiation of the main noradrenergic center of the brain. Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.
Background References:
  • Stanke M, Junghans D, Geissen M, et al. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. Development 1999; 126:4087-94
  • Young HM, Ciampoli D, Hsuan J, et al. Expression of Ret-, p75(NTR)-, Phox2a-, Phox2b-, and tyrosine hydroxalase-immunoreactivity by undifferentiated neural crest-derived cells and different classes of enteric neurons in the embryonic mouse gut. Dev. Dyn. 1999; 216:137-52.
  • Pattyn A, Morin X, Cremer H, et al. The homeobox gene Phox2b is essential for the development of autonomic neural crest derivatives. Nature 1999; 399:366-70.
  • Coppola E, d'Autr?aux F, Rijli FM, et al. Ongoing roles of Phox2 homeodomain transcription factors during neuronal differentiation. Development 2010; 137:4211-20
Buffer:
PHOX2B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (75%), Bovine: (79%)
Immunogen:
PHOX2B antibody was raised against a 15 amino acid synthetic peptide near the center of human PHOX2B.

The immunogen is located within amino acids 130 - 180 of PHOX2B.
NCBI Gene ID #:
8929
NCBI Official Name:
paired-like homeobox 2b
NCBI Official Symbol:
PHOX2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003915
Protein GI Number:
12707580
Purification:
PHOX2B Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
PHOX2B antibody is predicted to not cross-react with other paired homeobox family members.
Swissprot #:
Q99453
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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