ZNF521 Antibody
| Code | Size | Price |
|---|
| PSI-6065-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6065-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Documents
Further Information
Additional Names:
ZNF521 Antibody: EHZF, Evi3, EHZF, LIP3, Zinc finger protein 521, Early hematopoietic zinc finger protein
Application Note:
ZNF521 antibody can be used for detection of ZNF521 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
ZNF521 Antibody: The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
Background References:
- Warming S, Liu P, Suzuki T, et al. Evi3, a common retroviral integration site in murine B-cell lymphoma, encodes a EBFAZ-related Kruppel-like zinc finger protein. Blood 2003; 101:1934-40.
- Matsubara E, Sakai I, Yamanouchi J, et al. The role of zinc finger protein 521/early hematopoietic zinc finger protein in erythroid cell differentiation. J. Biol. Chem. 2009; 284:3480-7.
- Wu M, Hesse E, Morvan F, et al. Zfp521 antagonizes Runx2, delays osteoblast differentiation in vitro, and promotes bone formation in vivo. Bone 2009; 44:528-36.
- Hesse E, Saito H, Kiviranta R, et al. Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity. J. Cell Biol. 2010; 191:1271-83.
Buffer:
ZNF521 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
ZNF521 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZNF521.
The immunogen is located within amino acids 1160 - 1210 of ZNF521.
The immunogen is located within amino acids 1160 - 1210 of ZNF521.
NCBI Gene ID #:
25925
NCBI Official Name:
zinc finger protein 521
NCBI Official Symbol:
ZNF521
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056276
Protein GI Number:
24308069
Purification:
ZNF521 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
ZNF521 antibody is human specific.
Swissprot #:
Q96K83
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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