PRC1 Antibody

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ProSci
Product Code: PSI-6821
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6821-0.02mg0.02mg£150.00
Quantity:
PSI-6821-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of PRC1 in human skeletal muscle tissue lysate with Prc1 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.
2 / 3
Immunohistochemistry of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 20 μg/mL.
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Western blot analysis of PRC1 in human skeletal muscle tissue lysate with Prc1 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.
Immunohistochemistry of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 2.5 μg/mL.
Immunofluorescence of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
PRC1 Antibody: ASE1, Protein regulator of cytokinesis 1
Application Note:
PRC1 antibody can be used for detection of Prc1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.
Background References:
  • Jiang W, Jimenez G, Wells NJ, et al. PRC1: a human mitotic spindle-associated Cdk substrate protein required for cytokinesis. Mol. Cell 1998; 2:877-85.
  • Pellman D, Bagget M, Tu H, et al. Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces Cerevisiae. J. Cell Biol. 1995; 130:1373-85.
  • Kurasawa Y, Earnshaw WC, Mochizuki Y, et al. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 2004; 23:3237-48.
  • Mollinari C, Kleman JP, Jiang W, et al. PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone. J. Cell Biol. 2002; 157:1175-86.
Buffer:
PRC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PRC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human PRC1.

The immunogen is located within amino acids 350 - 400 of PRC1.
NCBI Gene ID #:
9055
NCBI Official Name:
protein regulator of cytokinesis 1
NCBI Official Symbol:
PRC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003972
Protein GI Number:
4506039
Purification:
PRC1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.
Swissprot #:
O43663
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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