WDR5 Antibody
| Code | Size | Price |
|---|
| PSI-6823-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6823-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
WDR5 Antibody: SWD3, BIG-3, BIG3, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein
Application Note:
WDR5 antibody can be used for detection of WDR5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
WDR5 Antibody: WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR5, also known as BIG-3, is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development. WDR5 interacts with the pluripotency factor Oct4/POU5F1 and is required for the efficient formation of induced pluripotent stem (iPS) cells.
Background References:
- Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5.
- Gori F and Demay MB. BIG-3, a novel WD-40 repeat protein, is expressed in the developing growth plate and accelerates chondrocyte differentiation in vitro. Endocrinology 2004; 145:1050-4.
- Gori F, Friedman LG, and Demay MB. Wdr5, a WD-40 protein, regulates osteoblast differentiation during embryonic bone development. Dev. Biol. 2006; 295:498-506.
- Ang YS, Tsai SY, Lee DF, et al. Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network. Cell 2011; 145:183-97.
Buffer:
WDR5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%), Bovine: (100%)
Immunogen:
WDR5 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human WDR5.
The immunogen is located within the first 50 amino acids of WDR5.
The immunogen is located within the first 50 amino acids of WDR5.
NCBI Gene ID #:
11091
NCBI Official Name:
WD repeat domain 5
NCBI Official Symbol:
WDR5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060058
Protein GI Number:
16554627
Purification:
WDR5 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
WDR5 antibody is human specific. WDR5 antibody is predicted to not cross-react with other WDR family members.
Swissprot #:
P61964
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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