SDHD Antibody

Product Code: PSI-6847

Supplier: ProSci

Code	Size	Price

PSI-6847-0.02mg

0.02mg

£150.00

Quantity:

PSI-6847-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

1 / 3

Western blot analysis of SDHD in EL4 cell lysate with SDHD antibody at (A) 1 and (B) 2 μg/mL.

2 / 3

Immunocytochemistry of SDHD in EL4 cells with SDHD antibody at 2.5 μg/mL.

3 / 3

Immunofluorescence of SDHD in EL4 cells with SDHD antibody at 20 μg/mL.

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Documents

Further Information

Additional Names:

SDHD Antibody: TRAP1, TRAP-1, SDH4, CII-4, CybS

Application Note:

SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Background:

SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.

Background References:

Baysal BE, Ferrell RE, Willett-Brozick JE, et al. Mutations in SDHD, a mitochondrial complex II gene, in hereditary paraganglioma. Science 2000; 287:848-51.
Saraste M. Oxidative phosphorylation at the fin de siecle. Science 1999; 283:1488-93.
Knudson AG. Genetics of human cancer. Annu. Rev. Genet. 1986; 20:231-51.
Gimenez-Roqueplo AP, Favier J, Rustin P, et al. The R22X mutation of the SDHD gene in hereditary paraganglioma abolishes the enzymatic activity of Complex II in the mitochondrial respiratory chain and activates the hypoxia pathway. Am. J. Hum. Genet. 2001; 69:1186-97.

Buffer:

SDHD Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%), Pig: (80%)

Immunogen:

SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD.

The immunogen is located within amino acids 30 - 80 of SDHD.

NCBI Gene ID #:

9392

NCBI Official Name:

succinate dehydrogenase complex, subunit D, integral membrane protein

NCBI Official Symbol:

SDHD

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

NP_002993

Protein GI Number:

4506865

Purification:

SDHD Antibody is affinity chromatography purified via peptide column.

Research Area:

Cancer,Cell Cycle

SPECIFICITY:

At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.

Swissprot #:

O14521

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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