ZNF521 Antibody
| Code | Size | Price |
|---|
| PSI-6859-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6859-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ZNF521 Antibody: EHZF, Evi3, EHZF, LIP3, Zinc finger protein 521, Early hematopoietic zinc finger protein
Application Note:
ZNF521 antibody can be used for detection of ZNF521 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ZNF521 Antibody: The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
Background References:
- Warming S, Liu P, Suzuki T, et al. Evi3, a common retroviral integration site in murine B-cell lymphoma, encodes a EBFAZ-related Kruppel-like zinc finger protein. Blood 2003; 101:1934-40.
- Matsubara E, Sakai I, Yamanouchi J, et al. The role of zinc finger protein 521/early hematopoietic zinc finger protein in erythroid cell differentiation. J. Biol. Chem. 2009; 284:3480-7.
- Wu M, Hesse E, Morvan F, et al. Zfp521 antagonizes Runx2, delays osteoblast differentiation in vitro, and promotes bone formation in vivo. Bone 2009; 44:528-36.
- Hesse E, Saito H, Kiviranta R, et al. Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity. J. Cell Biol. 2010; 191:1271-83
Buffer:
ZNF521 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (88%)
Immunogen:
ZNF521 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521.
The immunogen is located within the first 50 amino acids of ZNF521.
The immunogen is located within the first 50 amino acids of ZNF521.
NCBI Gene ID #:
25925
NCBI Official Name:
zinc finger protein 521
NCBI Official Symbol:
ZNF521
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 144 kDa
Observed: 145 kDa
Observed: 145 kDa
Protein Accession #:
NP_056276
Protein GI Number:
24308069
Purification:
ZNF521 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
ZNF521 antibody is human specific.
Swissprot #:
Q96K83
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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