LEMD2 Antibody
| Code | Size | Price |
|---|
| PSI-6891-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6891-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
LEMD2 Antibody: NET25, dJ482C21.1, LEM domain-containing protein 2, hLEM2
Application Note:
LEMD2 antibody can be used for detection of LEMD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
LEMD2 Antibody: The LEM (lamina-associated polypeptide-emerin-MAN1) domain is a motif shared by a group of lamin-interacting proteins of the inner nuclear membrane (INM) and nucleoplasm. One such protein, LEMD2 (LEM domain-containing protein 2), is a lamina-associated protein in the INM involved in nuclear structure organization, containing an N-terminal LEM motif, two transmembrane domains, and a MAN1-Src1p C-terminal (MSC) domain. LEMD2 is a truncated paralog of MAN1, lacking the MAN1-specific C-terminal RNA-recognition motif, but like MAN1 is required for myogenic differentiation. Mutations in LEMD2 are thought be among the possible causes of muscle disease.
Background References:
- Brachner A, Reipert S, Foisner R, et al. LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. J. Cell Sci. 2005; 118:5797-810
- Huber MD, Guan T, and Gerace L. Overlapping functions of nuclear envelope proteins NET25 (Lem2) and Emerin in regulation of extracellular signal-regulated kinase signaling in myoblast differentiation. Mol. Cell. Biol. 2009; 29:5718-28.
Buffer:
LEMD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LEMD2 antibody was raised against a 16 amino acid synthetic peptide near the center of human LEMD2.
The immunogen is located within amino acids 220 - 270 of LEMD2.
The immunogen is located within amino acids 220 - 270 of LEMD2.
NCBI Gene ID #:
221496
NCBI Official Name:
LEM domain containing 2
NCBI Official Symbol:
LEMD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_851853
Protein GI Number:
31044432
Purification:
LEMD2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
At least two isoforms of LEMD2 are known to exist; this antibody will detect only the longer isoform.
Swissprot #:
Q8NC56
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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