TRAP Antibody
| Code | Size | Price |
|---|
| PSI-6911-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6911-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TRAP Antibody: TRAP, SPENCDI, Tartrate-resistant acid phosphatase type 5, Tartrate-resistant acid ATPase, TR-AP
Application Note:
TRAP antibody can be used for detection of TRAP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TRAP Antibody: TRAP, also known as uteroferrin, is an iron containing, glycosylated, acid phosphatase. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. Along with the related protein ACP2, TRAP mediates the removal of mannose 6-phosphate residues from proteins targeted to lysosomes. TRAP is present in brain at low levels, but is expressed at a much higher level in liver.
Background References:
- Baumbach GA, Saunders PT, Bazer FW, et al. Uteroferrin has N-apsaragine-linked high mannose-type oligosaccharaides that contain mannose 6-phosphate. Proc. Natl. Acad. Sci. USA 1984; 81:2985-9.
- Sun P, Sleat DE, Lecocq M, et al. Acid phosphatase 5 is responsible for removing the mannose 6-phosphate recognition marker from lysosomal proteins. Proc. Natl. Acad. Sci. USA 2004; 105:16590-5.
- Makypridi G, Damme M, Muller-Loennies S, et al. Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5. Mol. Cell Biol. 2012; 32:774-82.
Buffer:
TRAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (94%), Rat: (89%), Rabbit: (88%)
Immunogen:
TRAP antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human TRAP.
The immunogen is located within the last 50 amino acids of TRAP.
The immunogen is located within the last 50 amino acids of TRAP.
NCBI Gene ID #:
54
NCBI Official Name:
acid phosphatase 5, tartrate resistant
NCBI Official Symbol:
ACP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001104505
Protein GI Number:
161377453
Purification:
TRAP Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
P13686
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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