VAMP7 Antibody

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ProSci
Product Code: PSI-6929
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6929-0.02mg0.02mg£150.00
Quantity:
PSI-6929-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunofluorescence of VAMP7 in human lung tissue with VAMP7 antibody at 20 μg/mL.
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Western blot analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunofluorescence of VAMP7 in human lung tissue with VAMP7 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
VAMP7 Antibody: SYBL1, TIVAMP, VAMP-7, TI-VAMP, SYBL1, Vesicle-associated membrane protein 7, Synaptobrevin-like protein 1
Application Note:
VAMP7 antibody can be used for detection of VAMP7 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
VAMP7 Antibody: VAMP7 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family, localizing to late endosomes and lysosomes. VAMP7 is thought to mediate the fusion of endosomes to their target lysosomes as well as other exocytosis events during phagocytosis and neuritogenesis. VAMP7 interacts with the VPS9 ankyrin repeat protein VARP, a protein that localizes to early endosomes and thought to regulate endosome dynamics. Together with CD82, VAMP7 can modulate the signaling of EGFR by regulating its endocytosis from the plasma membrane.
Background References:
  • Galli T, Zahraoui A, Vaidyanathan VV, et al. A novel tetanus neurotoxin-insensitive vesicle-associated membrane protein in SNARE complexes of the apical plasma membrane of epithelial cells. Mol. Biol. Cell 1998; 9:1437-48.
  • Wang Y and Tang BL. SNAREs in neurons - beyond synaptic vesicle exocytosis. Mol. Membr. Biol. 2006; 23:377-84.
  • Advani RJ, Yang B, Prekeris R, et al. VAMP-7 mediates vesicular transport from endosomes to lysosomes. J. Cell Biol. 1999; 146:765-75.
  • Burgo A, Sotirakis E, Simmler MC, et al. Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner in neurite growth. EMBO Rep. 2009; 10:1117-24.
Buffer:
VAMP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%)
Immunogen:
VAMP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human VAMP7.

The immunogen is located within amino acids 20 - 70 of VAMP7.
NCBI Gene ID #:
6845
NCBI Official Name:
vesicle-associated membrane protein 7
NCBI Official Symbol:
VAMP7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 24, 29 kDa

Observed: 32 kDa
Protein Accession #:
NP_001172112
Protein GI Number:
297747292
Purification:
VAMP7 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
At least three isoforms of VAMP7 are known to exist; this antibody will detect the two longest isoforms. This antibody is predicted to not cross-react with other Ras-related proteins.
Swissprot #:
P51809
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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