ZF21 Antibody
| Code | Size | Price |
|---|
| PSI-6935-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6935-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ZF21 Antibody: ZF21, Zinc finger FYVE domain-containing protein 21, ZF21
Application Note:
ZF21 antibody can be used for detection of ZF21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ZF21 Antibody: ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase), a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.
Background References:
- Uekita T, Gotoh I, Kinoshita T, et al. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. J. Biol. Chem. 2004; 279:12734-43.
- Nagano M, Hoshino D, Sakamoto T, et al. ZF21 protein regulates cell adhesion and motility. J. Biol. Chem. 2010; 285:21013-22.
- Nagano M, Hoshino D, Koshiba S, et al. ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain. J. Biol. Chem. 2011; 286:31598-609.
Buffer:
ZF21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
ZF21 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZF21.
The immunogen is located within the first 50 amino acids of ZF21.
The immunogen is located within the first 50 amino acids of ZF21.
NCBI Gene ID #:
79038
NCBI Official Name:
zinc finger, FYVE domain containing 21
NCBI Official Symbol:
ZFYVE21
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001185882
Protein GI Number:
312147341
Purification:
ZF21 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least two isoforms of ZF21 are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q9BQ24
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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