Caspase-3 Antibody
| Code | Size | Price |
|---|
| PSI-3445-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3445-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Documents
Further Information
Additional Names:
Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3
Application Note:
Caspase-3 antibody can be used for detection of Caspase-3 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
Caspase-3 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9.
Background References:
- Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74
- Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
- Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
- Slee EA, Adrain C, and Martin SJ. Serial killers: ordering caspase activation events in apoptosis. Cell Death Diff. 1999; 6:1067-74.
Buffer:
Caspase-3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Rabbit: (100%), Rat: (100%), Mouse: (87%), Bovine: (92%)
Immunogen:
Caspase-3 antibody was raised against a 17 amino acid synthetic peptide near the center of human Caspase-3.
The immunogen is located within amino acids 70 - 120 of Caspase-3.
The immunogen is located within amino acids 70 - 120 of Caspase-3.
NCBI Gene ID #:
836
NCBI Official Name:
caspase 3, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
NP_004337
Protein GI Number:
14790119
Purification:
Caspase-3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
At least two isoforms of Caspase-3 are known to exist.
Swissprot #:
P42574
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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