TCIRG1 Antibody

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ProSci
Product Code: PSI-6973
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6973-0.02mg0.02mg£150.00
Quantity:
PSI-6973-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of TCIRG1 in EL4 cell lysate with TCIRG1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Western blot analysis of TCIRG1 in EL4 cell lysate with TCIRG1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.

Documents

Further Information

Additional Names:
TCIRG1 Antibody: a3, Stv1, Vph1, Atp6i, OC116, OPTB1, TIRC7, ATP6N1C, ATP6V0A3, OC-116kDa, V-type proton ATPase 116 kDa subunit a isoform 3, Osteoclastic proton pump 116 kDa subunit, V-ATPase 116 kDa isoform a3
Application Note:
TCIRG1 antibody can be used for detection of TCIRG1 by Western blot at 0.5 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
TCIRG1 Antibody: The T-cell immune regulator 1 (TCIRG1) is the a3 subunit of the vacuolar-type proton transporting ATPase (V-ATPase), a proton pump in late endosomes and lysosomes that functions in the luminal acidification of these organelles. Genetic defects in the gene encoding this protein a responsible for a severe form of autosomal recessive osteopetrosis, a condition that leads to increased bone density, decreased bone strength, and inflammation in bone tissues. TCIRG1 is also required for the normal secretion of insulin and the bacteria-killing function of macrophages.
Background References:
  • Toyomura T, Oka T, Yamaguchi C, et al. Three subunit a isoforms of mouse vacuolar H+-ATPase. Preferential expression of the a3 isoform during osteoclast differentiation. J. Biol. Chem. 2000; 275:8760-5
  • Kornak U, Schulz A, Friedrich W, et al. Mutations in the a3 subunit of the vacuolar H+-ATPase cause malignant osteopetrosis. Hum. Mol. Genet. 2000; 9:2059-63.
  • Sun-Wada GH, Toyomura T, Kawamura N, et al. The a3 isoform of V-ATPase regulates insulin secretion from pancreatic b-cells. J. Cell Sci. 2006; 119:4531-40.
  • Sun-Wada GH, Tabata H, Kawamura N, et al. Direct recruitment of H+-ATPase from lysosomes for phagocytic acidification. J. Cell Sci. 2009; 122:2504-13.
Buffer:
TCIRG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TCIRG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCIRG1.

The immunogen is located within amino acids 70 - 120 of TCIRG1.
NCBI Gene ID #:
10312
NCBI Official Name:
T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
NCBI Official Symbol:
TCIRG1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
91 kDa
Protein Accession #:
NP_006010
Protein GI Number:
19924145
Purification:
TCIRG1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform.
Swissprot #:
Q13488
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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