SELK Antibody
| Code | Size | Price |
|---|
| PSI-6975-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6975-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SELK Antibody: SelK, HSPC030, HSPC297, Selenoprotein K, SelK
Application Note:
SELK antibody can be used for detection of SELK by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SELK Antibody: SELK is a selenoprotein, containing a selenocysteine residue at its active site. SELK is localized to the endoplasmic reticulum and is highly expressed in the heart, where it may function as an antioxidant. SELK can bind to proteins such as the ER-associated degradation (ERAD) components p97 ATPase, Derlin-1 and -2, and SelS as well as proteins in the oligosaccharyltransferase complex, suggesting that SELK may be involved in the Derlin-dependent ERAD of glycosylated misfolded proteins. SELK is also thought to be important in Ca2+ flux in immune cells, and is cleaved by m-calpain in resting macrophages. When these macrophages are activated through different Toll-like receptors, this cleavage is inhibited
Background References:
- Lu C, Qiu F, Zhou H, et al. Identification and characterization of selenoprotein K: An antioxidant in cardiomyocytes. FEBS Lett. 2006; 580:5189-97
- Shchedrina VA, Everly RA, Zhang Y, et al. Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic homeostasis. J. Biol. Chem. 2011; 286:42937-48.
- Huang Z, Hoffmann FW, Norton RL, et al. Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages. J. Biol. Chem. 2011; 286:34830-8.
Buffer:
SELK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%)
Immunogen:
SELK antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SELK.
The immunogen is located within the last 50 amino acids of SELK.
The immunogen is located within the last 50 amino acids of SELK.
NCBI Gene ID #:
58515
NCBI Official Name:
selenoprotein K
NCBI Official Symbol:
SELK
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
10 kDa
Protein Accession #:
NP_067060
Protein GI Number:
25014099
Purification:
SELK Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q9Y6D0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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