TOX Antibody
| Code | Size | Price |
|---|
| PSI-6987-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6987-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TOX Antibody: TOX1, KIAA0808, Thymocyte selection-associated high mobility group box protein TOX, Thymus high mobility group box protein TOX
Application Note:
TOX antibody can be used for detection of TOX by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TOX Antibody: TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.
Background References:
- Wilkinson B, Chen JY, Han P, et al. TOX: an HMG box protein implicated in the regulation of thymocyte selection. Nat. Immunol. 2002; 3:272-80.
- Saito T and Watanabe N. Positive and negative thymocyte selection. Crit. Rev. Immunol. 1998; 18:359-70
- Aliahmad P, O'Flaherty E, Han P, et al. TOX provides a link between calcineurin activation and CD8 lineage commitment. J. Exp. Med. 2004; 199:1089-99.
- Aliahmad P, Kadavallore A, de la Torre B, et al. TOX is required for development of the CD4 T cell lineage gene program. J. Immunol. 2011; 187:5931-40.
Buffer:
TOX Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (76%)
Immunogen:
TOX antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOX.
The immunogen is located within amino acids 130 - 180 of TOX.
The immunogen is located within amino acids 130 - 180 of TOX.
NCBI Gene ID #:
9760
NCBI Official Name:
thymocyte selection-associated high mobility group box protein TOX
NCBI Official Symbol:
TOX
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
NP_055544
Protein GI Number:
7662322
Purification:
TOX Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
No alternatively spliced transcript variants have been observed.
Swissprot #:
O94900
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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