ATG2B Antibody

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ProSci
Product Code: PSI-7005
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7005-0.02mg0.02mg£150.00
Quantity:
PSI-7005-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of ATG2B in K562 cell lysate with ATG2B antibody at (A) 1 and (B) 2 μg/ml
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Immunofluorescence of ATG2B in K562 cells with ATG2B antibody at 20 μg/mL.
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Western blot analysis of ATG2B in K562 cell lysate with ATG2B antibody at (A) 1 and (B) 2 μg/ml
Immunofluorescence of ATG2B in K562 cells with ATG2B antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
ATG2B Antibody: C14orf103, C14orf103, Autophagy-related protein 2 homolog B
Application Note:
ATG2B antibody can be used for detection of ATG2B by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ATG2B Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is ATG2B, one of two homologs of ATG2 that is essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Relatively high rates of ATG2B mutations were observed in gastric and colorectal carcinomas, suggesting that deregulating the autophagy process may contribute to cancer development.
Background References:
  • Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
  • Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
  • Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
  • Velikkakath AK, Nishimura T, Oita E, et al. Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol. Biol. Cell 2012; 23:896-909.
Buffer:
ATG2B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATG2B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATG2B.

The immunogen is located within amino acids 450 - 500 of ATG2B.
NCBI Gene ID #:
55102
NCBI Official Name:
autophagy-related protein 2 homolog B
NCBI Official Symbol:
ATG2B
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
229 kDa
Protein Accession #:
NP_060506
Protein GI Number:
118197272
Purification:
ATG2B Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
ATG2B antibody is predicted to not cross-react with other ATG2A.
Swissprot #:
Q96BY7
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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