TET3 Antibody

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ProSci
Product Code: PSI-7013
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7013-0.02mg0.02mg£150.00
Quantity:
PSI-7013-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of TET3 in human brain tissue with TET3 antibody at 2.5 μg/ml.
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Immunofluorescence of TET3 in human brain tissue with TET3 antibody at 20 μg/mL.
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Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TET3 in human brain tissue with TET3 antibody at 2.5 μg/ml.
Immunofluorescence of TET3 in human brain tissue with TET3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
TET3 Antibody: hCG_40738, KIAA0401, Methylcytosine dioxygenase TET3
Application Note:
TET3 antibody can be used for detection of TET3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TET3 Antibody: TET3, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine and is most abundantly expressed in hematopoietic cells. Unlike the related TET2 protein, mutations in TET3 have not been observed in any myeloid malignancies. TET3 has been shown to be involved in the demethylation of zygotic DNA before the first mitosis and has been suggested to be involved in the epigenetic reprogramming of the zygotic paternal DNA following natural fertilization and may also contribute to somatic cell nuclear reprogramming during animal cloning.
Background References:
  • Langemeijer SM, Kuiper RP, Berends M, et al. Acquired mutations in TET2 are common in myelodysplastic syndromes. Nat. Genet. 2009; 41:838-42.
  • Schaub FX, Looser R, Li S, et al. Clonal analysis of TET2 and JAK2 mutations suggests that TET2 can be a late event in the progression of myeloproliferative neoplasms. Blood 2011; 115:2003-7.
  • Langemeijer SMC, Aslanyan MG, and Jansen JH. TET proteins in malignant hematopoiesis. Cell Cycle 2009; 4044-8.
  • Gu TP, Guo F, Yang H, et al. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 2011; 477:606-10.
Buffer:
TET3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TET3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TET3.

The immunogen is located within amino acids 1520 - 1570 of TET3.
NCBI Gene ID #:
200424
NCBI Official Name:
TET methylcytosine dioxygenase 3
NCBI Official Symbol:
TET3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
183 kDa
Protein Accession #:
NP_001274420
Protein GI Number:
149944516
Purification:
TET3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least three isoforms of TET3 are known to exist. TET3 antibody is predicted to not cross react with other TET proteins.
Swissprot #:
O43151
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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