PHLPP1 Antibody
| Code | Size | Price |
|---|
| PSI-7019-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7019-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PHLPP1 Antibody: SCOP, PHLPP, PLEKHE1, KIAA0606, SCOP, PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, PH domain-containing family E member 1
Application Note:
PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
Background References:
- Brognard J and Newton AC. PHLiPPing the switch on Akt and protein kinase C signaling. Trends Endocrinol. Metab. 2008; 19:223-30.
- Gao T, Furnari F and Newton AC. PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol. Cell 2005; 18:13-24.
- Brognard J, Sierecki E, Gao T, et al. PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol. Cell 2007; 25:917-31
- Andreozzi F, Procopio C, Greco A, et al. Increased levels of the Akt-specific phosphatase PH domain leucine-rich repeat protein phosphatase (PHLPP)-1 in obese participants are associated with insulin resistance. Diabetologia 2011; 54:1879-87.
Buffer:
PHLPP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1.
The immunogen is located within amino acids 250 - 300 of PHLPP1.
The immunogen is located within amino acids 250 - 300 of PHLPP1.
NCBI Gene ID #:
23239
NCBI Official Name:
PH domain and leucine rich repeat protein phosphatase 1
NCBI Official Symbol:
PHLPP1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
189 kDa
Protein Accession #:
NP_919431
Protein GI Number:
291219891
Purification:
PHLPP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.
Swissprot #:
O60346
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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