CLAUDIN4 Antibody

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ProSci
Product Code: PSI-7025
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7025-0.02mg0.02mg£150.00
Quantity:
PSI-7025-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLAUDIN4, 7025 (1 μg/mL), CLAUDIN4, 24-028 (1 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLAUDIN4, 7025 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Colon Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLAUDIN4, 7025 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Colon Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLAUDIN4, 7025 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLAUDIN4, 7025 (1 μg/mL), CLAUDIN4, 24-028 (1 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLAUDIN4, 7025 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Colon Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLAUDIN4, 7025 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Colon Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CLAUDIN4, 7025 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
CLAUDIN4 Antibody: CPER, CPE-R, CPETR, CPETR1, WBSCR8, hCPE-R, CPER, Claudin-4, Clostridium perfringens enterotoxin receptor
Application Note:
WB: 0.5-2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CLAUDIN4 Antibody: CLAUDIN4 is an integral membrane protein belonging to the claudin family, a family of cellular adhesion molecules that are components of tight junctions. CLAUDIN4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The CLAUDIN4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. CLAUDIN4 is frequently overexpressed in ovarian and other epithelial cancers.
Background References:
  • Mitic et al. Protein Sci. 2003; 12:218-27.
  • Paperna et al. Genomics 1998; 54:453-9.
  • Morin. Cancer Res. 2005; 65:9603-6.
Buffer:
CLAUDIN4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%), Mouse (94%) and Rat (88%).
Immunogen:
Anti-CLAUDIN4 antibody (7025) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human CLAUDIN4.

The immunogen is located within the last 50 amino acids of CLAUDIN4.
ISOFORMS:
Human CLAUDIN4 has one isoform (209aa, 22kD). Mouse CLAUDIN4 has one isoform (210aa, 22kD) and Rat CLAUDIN4 also has one isoform (210aa, 22kD). 7025 can detect human, mouse and rat.
NCBI Gene ID #:
1364
NCBI Official Name:
claudin 4
NCBI Official Symbol:
CLDN4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 22kD

Observed: 22 kD
Protein Accession #:
NP_001296
Protein GI Number:
4502877
Purification:
CLAUDIN4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least four isoforms of CLAUDIN4 are known to exist; CLAUDIN4 antibody will detect all four isoforms
Swissprot #:
O14493
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar CLAUDIN4 expression profile in human cell lines detected by two independent anti-CLAUDIN4 antibodies that recognize different epitopes, 7025 against C-terminus domain and 24-028 against C-terminus domain.  CLAUDIN4 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

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