CENPW Antibody

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ProSci
Product Code: PSI-6943
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6943-0.02mg0.02mg£150.00
Quantity:
PSI-6943-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of CENPW in HeLa cell lysate lysate with CENPW antibody at (A) 0.5 and (B) 1 μg/mL.
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Western blot analysis of CENPW in HeLa cell lysate lysate with CENPW antibody at (A) 0.5 and (B) 1 μg/mL.

Documents

Further Information

Additional Names:
CENPW Antibody: CUG2, CENP-W, C6orf173, CUG2, Centromere protein W, Cancer-up-regulated gene 2 protein
Application Note:
CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
CENPW Antibody: CENPW was initially identified as a gene that was upregulated in multiple cancers, and whose overexpression in mouse fibroblast cells gave rise to distinct cancer-specific phenotypes. It was later found to be a nuclear protein that associated with CENPT, a component of CENPA nucleosome complex in the centromere, and is required for proper kinetochore function. CENPW also specifically interacts with the nucleolar phosphoprotein nucleophosmin, also known as B23 It has been suggested that nucleophosmin functions in the assembly of the kinetochore by interacting with CENPW during interphase. Overexpression of CENPW in the SKOV-3 human ovarian cancer cell line as well as in the zebrafish embryo led to apoptosis, suggesting that high levels of CENPW induces apoptotic cell death.
Background References:
  • Lee S, Gang J, Jeon SB, et al. molecular cloning and functional analysis of a novel oncogene, cancer-upregulated gene 2 (CUG2). Biochem. Biophys. Res. Commun. 2007; 360:633-9.
  • Kim H, Lee M, Lee S, et al. Cancer-upregulated gene 2 (CUG2), a new component of centromere complex, is required for kinetochore function. Mol. Cells 2009; 27:697-701.
  • Chun Y, Park B, Koh W, et al. New centromeric component CENT-W is an RNA-associated nuclear matrix protein that interacts with nucleophosmin/B23 protein. J. Biol. Chem. 2011; 286:42758-69.
  • Lee S, Koh W, Kim HT, et al. Cancer-upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV-3 cells. Cell Biochem. Funct. 2010; 28:461-8.
Buffer:
CENPW Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CENPW antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CENPW.

The immunogen is located within the first 50 amino acids of CENPW.
NCBI Gene ID #:
387103
NCBI Official Name:
centromere protein W
NCBI Official Symbol:
CENPW
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
kDa
Protein Accession #:
NP_001012525
Protein GI Number:
60302883
Purification:
CENPW Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
At least three isoforms of CENPW are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q5EE01
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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