DDX60 Antibody
| Code | Size | Price |
|---|
| PSI-6945-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6945-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
DDX60 Antibody: Probable ATP-dependent RNA helicase DDX60, DEAD box protein 60
Application Note:
DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
DDX60 Antibody: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.
Background References:
- Miyashita M, Oshiumi H, Matsumoto M, et al. DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling. Mol. Cell. Biol. 2011; 31:3802-19.
Buffer:
DDX60 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.
The immunogen is located within amino acids 1140 - 1190 of DDX60.
The immunogen is located within amino acids 1140 - 1190 of DDX60.
NCBI Gene ID #:
55601
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
NCBI Official Symbol:
DDX60
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 125, 185, 188 kDa
Observed: 190 kDa
Observed: 190 kDa
Protein Accession #:
NP_060101
Protein GI Number:
222831595
Purification:
DDX60 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q8IY21
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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