WIPI1 Antibody

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ProSci
Product Code: PSI-7041
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7041-0.02mg0.02mg£150.00
Quantity:
PSI-7041-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of WIPI1 in rat colon tissue lysate with WIPI1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of WIPI1 in human colon tissue with WIPI1 antibody at 20 μg/mL.
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Western blot analysis of WIPI1 in rat colon tissue lysate with WIPI1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of WIPI1 in human colon tissue with WIPI1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
WIPI1 Antibody: ATG18, ATG18A, WIPI49, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog, WIPI-1
Application Note:
WIPI1 antibody can be used for detection of Wipi1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
WIPI1 Antibody: WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as WIPI1, ATG18 or WIPI49, is thought to play a role in autophagy and may regulate protein trafficking in certain recycling pathways. It contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles and the trans-Golgi network. It is ubiquitously expressed with highest expression in heart, testis, placenta, pancreas and skeletal muscle. WIPI1 is upregulated in a variety of tumors, suggesting a role in carcinogenesis.
Background References:
  • Proikas-Cezanne T and Robenek H. Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J. Cell. Mol. Med. 2011; 15:2007-10.
  • Jeffries TR, Dove SK, Michell RH, et al. PtdIns-specific MPR pathway association of a novel WD40 repeat protein, WIPI49. Mol. Biol. Cell. 2004;15:2652-63.
  • Proikas-Cezanne T, Waddell S, Gaugel A, et al. WIPI-1 (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy. Oncogene 2004; 23:9314-25.
  • Proikas-Cezanne T, Ruckerbauer S, Stierhof YD, et al. Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy. FEBS Lett. 2007; 581:3396-404.
Buffer:
WIPI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
WIPI1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WIPI1.

The immunogen is located within amino acids 350 - 400 of WIPI1.
NCBI Gene ID #:
55062
NCBI Official Name:
WD repeat domain, phosphoinositide interacting 1
NCBI Official Symbol:
WIPI1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
NP_060453
Protein GI Number:
157388939
Purification:
WIPI1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
At least two isoforms of WIPI1 are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q5MNZ9
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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