LNPEP Antibody

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ProSci
Product Code: PSI-7055
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7055-0.02mg0.02mg£150.00
Quantity:
PSI-7055-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of LNPEP in human lung tissue lysate with LNPEP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunofluorescence of LNPEP in human lung tissue with LNPEP antibody at 20 μg/mL.
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Western blot analysis of LNPEP in human lung tissue lysate with LNPEP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of LNPEP in human lung tissue with LNPEP antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
LNPEP Antibody: CAP, IRAP, PLAP, P-LAP, OTASE, Leucyl-cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Cystinyl aminopeptidase
Application Note:
LNPEP antibody can be used for detection of LNPEP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
LNPEP Antibody: LNPEP, also known as insulin-responsive aminopeptidase (IRAP), was initially identified as an abundant protein that is associated with specialized endosomes termed GLUT4 storage vesicles (GSVs) in adipocytes. The luminal domain of LNPEP contains an aminopeptidase activity that is involved in peptide hormone processing and antigenic peptide processing. LNPEP is also a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to the GSVs.
Background References:
  • Keller SR, Scott HM, Mastick CC, et al. Cloning and characterization of a novel insulin-regulated membrane aminopeptidase from Glut4 vesicles. J. Biol. Chem. 1995; 270:23612-8.
  • Wallis MG, Lankford MF, and Keller SR. Vasopressin is a physiological substrate for the insulin-regulated aminopeptidase IRAP. Am. J. Physiol. Endocrinol. Metab. 2007; 293:E1092-102.
  • Saveanu L, Carroll O, Weimershaus M, et al. IRAP identifies an endosomal compartment required for MHC class I cross-presentation. Science 2009; 325:213-7.
  • Jordens I, Molle D, Xiong W, et al. Insulin-regulated aminopeptidase is a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to specialized insulin-regulated vesicles. Mol. Biol. Cell 2010; 21:2034-44.
Buffer:
LNPEP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (73%), Rat: (73%)
Immunogen:
LNPEP antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human LNPEP.

The immunogen is located within amino acids 900 - 950 of LNPEP.
NCBI Gene ID #:
4012
NCBI Official Name:
leucyl/cystinyl aminopeptidase
NCBI Official Symbol:
LNPEP
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
113 kDa
Protein Accession #:
NP_005566
Protein GI Number:
61742777
Purification:
LNPEP Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.
Swissprot #:
Q9UIQ6
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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