PLA1A Antibody

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ProSci
Product Code: PSI-7059
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7059-0.02mg0.02mg£150.00
Quantity:
PSI-7059-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of PLA1A in human kidney tissue lysate with PLA1A antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of PLA1A in human kidney tissue with PLA1A antibody at 20 μg/mL.
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Western blot analysis of PLA1A in human kidney tissue lysate with PLA1A antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of PLA1A in human kidney tissue with PLA1A antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
PLA1A Antibody: PSPLA1, PS-PLA1, NMD, PSPLA1, Phospholipase A1 member A, Phosphatidylserine-specific phospholipase A1
Application Note:
PLA1A antibody can be used for detection of PLA1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PLA1A Antibody: PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine (PS) in liposomes and can also hydrolyze PS in apoptotic cells and activate platelets where the resulting 2-acyl-lysophosphatidylserine acts as a lipid mediator for mast cells, T cells, and neural cells, suggesting that a major function of PLA1A may be the production of lysophospholipid mediators. PLA1A is upregulated in rat peripheral blood cells bearing long-term surviving cardiac allograft. PLA1A is also expressed in human THP-1-derived macrophages and this expression is upregulated in cells treated with lipopolysaccharide, a TLR4 ligand. This upregulation is inhibited with corticosteroids, which are often used at high dosages to suppress chronic allograft rejection.
Background References:
  • Sato T, Aoki J, Nagai Y, et al. Serine phospholipid-specific phospholipase A that is secreted from activated platelets. A new member of the lipase family. J. Biol. Chem. 1997; 272:2192-8.
  • Aoki J, Nagai Y, Hosono H, et al. Structure and function of phosphatidylserine-specific phospholipase A1. Biochim. Biophys. Acta 2002; 1582:26-32.
  • Hosono H, Homma M, Ogasawa Y, et al. Expression of phosphatidylserine-specific phospholipase A(1) mRNA in human THP-1-derived macrophages. Cell Transplant. 2010; 19:759-64.
Buffer:
PLA1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (75%), Bovine: (71%)
Immunogen:
PLA1A antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human PLA1A.

The immunogen is located within amino acids 380 - 430 of PLA1A.
NCBI Gene ID #:
51365
NCBI Official Name:
phospholipase A1 member A
NCBI Official Symbol:
PLA1A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
NP_056984
Protein GI Number:
7706661
Purification:
PLA1A Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
At least three isoforms of PLA1A are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q53H76
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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