CITED2 Antibody

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ProSci
Product Code: PSI-7067
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7067-0.02mg0.02mg£150.00
Quantity:
PSI-7067-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of CITED2 in Jurkat cell lysate with CITED2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of CITED2 in Jurkat cells with CITED2 antibody at 20 μg/mL.
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Western blot analysis of CITED2 in Jurkat cell lysate with CITED2 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of CITED2 in Jurkat cells with CITED2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
CITED2 Antibody: ASD8, MRG1, VSD2, MRG-1, P35SRJ, MSG-related protein 1
Application Note:
CITED2 antibody can be used for detection of CITED2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CITED2 Antibody: CITED2 was initially identified as a potential transcriptional activator with significant homology to MSG1, a melanocyte-specific nuclear protein. CITED2 interacts with the histone acetyltransferase p300/CBP and acts as a coactivator to several DNA-binding transcription factors such as HIF-1 and AP-2. CITED2 also controls proliferation of mouse embryonic fibroblasts via the polycomb group genes Bmi-1 and Mel18 and the tumor suppressor Ink4a/Arf. CITED2 has also been found to be an essential regulator of adult hematopoietic stem cells (HSCs), with conditional deletion of CITED2 in the adult mouse resulting in the loss of HSCs, multilineage bone marrow failure and increased lethality.
Background References:
  • Shioda T, Fenner MH, and Isselbacher KJ. MSG1 and its related protein MRG1 share a transcription activating domain. Gene 1997; 204:235-41.
  • Bhattachatya S, Michels CL, Leung MK, et al. Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Genes Dev. 1999; 13:64-75.
  • Bamforth SD, Braganca J, Eloranta JJ, et al. Cardiac malformations, adrenal agenesis, neural crest defects and exencephaly in mice lacking Cited2, a new Tfap2 co-activator. Nat. Genet. 2001; 29:469-74.
  • Kranc KR, Bamforth SD, Braganca J, et al. Transcriptional coactivator Cited2 induces Bmi1 and Mel18 and controls fibroblast proliferation via Ink4a/ARF. Mol. Cell. Biol. 2003; 23:7658-66.
Buffer:
CITED2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CITED2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CITED2.

The immunogen is located within the first 50 amino acids of CITED2.
NCBI Gene ID #:
10370
NCBI Official Name:
Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
NCBI Official Symbol:
CITED2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
NP_001161861
Protein GI Number:
270288756
Purification:
CITED2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
Multiple isoforms of CITED2 are known to exist.
Swissprot #:
Q99967
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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