BATF Antibody

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ProSci
Product Code: PSI-7069
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7069-0.02mg0.02mg£150.00
Quantity:
PSI-7069-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of BATF in rat spleen tissue lysate with BATF antibody at 1 μg/mL.
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Immunofluorescence of BATF in human spleen tissue with BATF antibody at 20 μg/mL.
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Western blot analysis of BATF in rat spleen tissue lysate with BATF antibody at 1 μg/mL.
Immunofluorescence of BATF in human spleen tissue with BATF antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
BATF Antibody: SFA2, B-ATF, BATF1, SFA-2, Basic leucine zipper transcriptional factor ATF-like, B-cell-activating transcription factor
Application Note:
BATF antibody can be used for detection of BATF by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BATF Antibody: BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos. BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense. In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.
Background References:
  • Dorsey MJ, Tae HJ, Sollenberger KG, et al. B-ATF: a novel human bZIP protein that associates with members of the AP-1 transcription factor family. Oncogene 1995; 11:2255-65.
  • Echlin DR, Tae HJ, Mitin N, et al. B-ATF functions as a negative regulator of AP-1 mediated transcription and blocks cellular transformation by Ras and Fos. Oncogene 2000; 19:1752-63.
  • Schraml BU, Hildner K, Ise W, et al. The AP-1 transcription factor Batf controls TH17 differentiation. Nature 2009; 460:405-9.
  • Ise W, Kohyama M, Schraml BU, et al. The transcription factor BATF controls the global regulators of class-switch recombination in both B cells and T cells. Nat. Immunol. 2011; 12:536-43.
Buffer:
BATF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
BATF antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BATF.

The immunogen is located within the first 50 amino acids of BATF.
NCBI Gene ID #:
10538
NCBI Official Name:
basic leucine zipper transcription factor, ATF-like
NCBI Official Symbol:
BATF
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
NP_006390
Protein GI Number:
5453563
Purification:
BATF Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
Multiple isoforms of BATF are known to exist. Despite its predicted molecular weight, BATF often migrates at a higher than expected molecular weight.
Swissprot #:
Q16520
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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