SAMSN Antibody

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ProSci
Product Code: PSI-7079
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7079-0.02mg0.02mg£150.00
Quantity:
PSI-7079-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of SAMSN in Hela cell lysate with SAMSN antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunocytochemistry of SAMSN1 in HeLa cells with SAMSN1 antibody at 2.5 μg/ml.
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Immunofluorescence of SAMSN1 in HeLa cells with SAMSN1 antibody at 5 μg/ml.
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Western blot analysis of SAMSN in Hela cell lysate with SAMSN antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of SAMSN1 in HeLa cells with SAMSN1 antibody at 2.5 μg/ml.
Immunofluorescence of SAMSN1 in HeLa cells with SAMSN1 antibody at 5 μg/ml.

Documents

Further Information

Additional Names:
SAMSN Antibody: SLy2, HACS1, NASH1, SASH2, SH3D6B, SAM domain-containing protein SAMSN-1, Hematopoietic adaptor containing SH3 and SAM domains 1
Application Note:
SAMSN antibody can be used for detection of SAMSN by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SAMSN Antibody: SAMSN (HACS1) is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains. It encodes a 441 amino acid protein that is differentially expressed in hematopoietic cells and malignancies including myeloid leukemia, lymphoma, and myeloma. SAMSN has restricted expression in human tissues. It has been shown to be up-regulated by B cell activation signals and is a participant in B cell activation and differentiation. SAMSN is an immunoinhibitory adaptor that might be a useful target for immune suppression therapy.
Background References:
  • Uchida T, Nakao A, Nakano N, et al. Identification of Nash1, a novel protein containing a nuclear localization signal, a sterile alpha motif, and an SH3 domain preferentially expressed in mast cells. Biochem. Biophys. Res. Commun. 2001; 288:137-41.
  • Claudio JO, Zhu YX, Benn SJ, et al. HACS1 encodes a novel SH3-SAM adaptor protein differentially expressed in normal and malignant hematopoietic cells. Oncogene 2001; 20:5373-7.
  • Zhu YX, Benn S, Li ZH, et al. The SH3-SAM adaptor HACS1 is up-regulated in B cell activation signaling cascades. J. Exp. Med. 2004; 200:737-47.
  • Wang D, Stewart AK, Zhuang L, et al. Enhanced adaptive immunity in mice lacking the immunoinhibitory adaptor Hacs1. FASEB J. 2010; 24:947-56.
Buffer:
SAMSN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%)
Immunogen:
Rabbit polyclonal SAMSN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human SAMSN.

The immunogen is located within the last 50 amino acids of SAMSN.
NCBI Gene ID #:
64092
NCBI Official Name:
SAM domain, SH3 domain and nuclear localization signals 1
NCBI Official Symbol:
SAMSN1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
NP_071419
Protein GI Number:
48762709
Purification:
SAMSN Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
At least two isoforms of SAMSN are known to exist.
Swissprot #:
Q9NSI8
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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