ACSL1 Antibody

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ProSci
Product Code: PSI-7081
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7081-0.02mg0.02mg£150.00
Quantity:
PSI-7081-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of ACSL1 expression in human liver tissue lysate with ACSL1 antibody at 0.25 μg/ml.
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Immunofluorescence of ACSL1 in human lung tissue with ACSL1 antibody at 20 μg/mL.
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Western blot analysis of ACSL1 expression in human liver tissue lysate with ACSL1 antibody at 0.25 μg/ml.
Immunofluorescence of ACSL1 in human lung tissue with ACSL1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
ACSL1 Antibody: ACS1, LACS, FACL1, FACL2, LACS1, LACS2, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, ACS1
Application Note:
ACSL1 antibody can be used for detection of ACSL1 by Western blot at 0.25 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ACSL1 Antibody: Long-chain acyl coenzyme A synthetase 1 (ACSL1) catalyzes the synthesis of acyl-CoA from long-chain fatty acids in an ATP-dependent manner. ACSL1 is a member of a family of long-chain acyl-CoA synthetases which differ in substrate preference, tissue expression, and subcellular localization. In mouse, ACSL1 is the major acyl-CoA enzyme in the heart, providing 60-90% of heart ATP. Loss of ACSL1 either globally or in heart ventricles resulted in impaired fatty acid oxidation, activation of the mammalian target of rapamycin (mTOR), and cardiac hypertrophy.
Background References:
  • Black PN and DiRusso CC. Transmembrane movement of exogenous long-chain fatty acids: proteins, enzymes, and vectorial esterification. Microbiol. Mol. Biol. Rev. 2003; 67:454-72.
  • Coleman RA, Lewin TM, Van Horn CG, et al. Do acyl-CoA synthetases regulate fatty acid entry into synthetic versus degradative pathways J. Nutr. 2002; 132:2123-6.
  • Clark H, Carling D, and Saggerson D. Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids. Eur. J. Biochem. 2004; 271:2215-24
  • Ellis JM, Mentock SM, DePetrillo MA, et al. Mouse cardiac acyl Coenzyme A synthetase 1 deficiency impairs fatty acid oxidation and induces cardiac hypertrophy. Mol. Cell. Biol. 2011; 31:1252-62.
Buffer:
ACSL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)
Immunogen:
Rabbit polyclonal ACSL1 antibody was raised against an 18 amino acid peptide near the center of human ACSL1.

The immunogen is located within amino acids 240 - 290 of ACSL1.
NCBI Gene ID #:
2180
NCBI Official Name:
acyl-CoA synthetase long-chain family member 1
NCBI Official Symbol:
ACSL1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 41, 58, 77 kDa

Observed: 45, 47 kDa?
Protein Accession #:
NP_001986
Protein GI Number:
40807491
Purification:
ACSL1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
At least three isoforms of ACSL1 are known to exist; this antibody will detect all three isoforms.
Swissprot #:
P33121
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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