POU3F2 Antibody
| Code | Size | Price |
|---|
| PSI-7085-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7085-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
POU3F2 Antibody: BRN2, OCT7, OTF7, OTF-7, POUF3, brn-2, oct-7, N-Oct3, BRN2, POU domain, class 3, transcription factor 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Brain-2
Application Note:
POU3F2 antibody can be used for detection of POU3F2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
POU3F2 Antibody: POU3F2, also commonly known as brain-2, is a member of a family of POU domain genes expressed in mouse brain and is thought to be involved in the development of the neocortex and establishment of neural cell lineage. Recent studies suggest that POU3F2 may be involved with the development of neurodegenerative diseases as well as tumor development and proliferation. Along with the neural-lineage-specific transcription factors ASCL1 and MYT1L, POU3F2 can convert fibroblasts to functional neurons in vitro, a form of artificial stem cells termed induced neuronal (iN) cells, suggesting that these cells may be useful in the treatment of neurodegenerative diseases.
Background References:
- Hara Y, Rovescalli AC, Kim Y, et al. Structure and evolution of four POU domain genes expressed in mouse brain. Proc. Natl. Acad. Sci. USA 1992; 89:3280-4.
- McEvilly RJ, de Diaz MO, Schonemann MD, et al. Transcriptional regulation of cortical neuron migration by POU domain factors. Science 2002; 295:1528-32.
- Huang YT, Iwamoto K, Kurosaki T, et al. The neuronal POU transcription factor Brn-2 interacts with Jab1, a gene involved in the onset of neurodegenerative diseases. Neurosci. Lett. 2005; 382:175-8
- Goodall J, Carreira S, Denat L, et al. Brn-2 represses microphthalmia-associated transcription factor expression and marks a distinct subpopulation of microphthalmia-associated transcription factor-negative melanoma cells. Cancer Res. 2008; 68:7788-94
Buffer:
POU3F2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal POU3F2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human POU3F2.
The immunogen is located within the last 50 amino acids of POU3F2.
The immunogen is located within the last 50 amino acids of POU3F2.
NCBI Gene ID #:
5454
NCBI Official Name:
POU class 3 homeobox 2
NCBI Official Symbol:
POU3F2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
NP_005595
Protein GI Number:
51702521
Purification:
POU3F2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least two isoforms are known to exist. This antibody will recognize both isoforms. POU3F2 antibody is predicted to not cross-react with other members of the POU domain class 3 family.
Swissprot #:
P20265
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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