LMX1B Antibody

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ProSci
Product Code: PSI-7089
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7089-0.02mg0.02mg£150.00
Quantity:
PSI-7089-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 μg/mL.
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Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
LMX1B Antibody: NPS1, LMX1.2, LIM homeobox transcription factor 1-beta
Application Note:
LMX1B antibody can be used for detection of LMX1B by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
LMX1B Antibody: The LIM homeobox transcription factor 1B (LMX1B) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1B regulates mid-hindbrain patterning; LMX1B-null mice embryos have a severe reduction in the number of midbrain dopaminergic neurons compared to wild-type. While LMX1B appears to be important for both the development and the survival of dopamine neurons, the related LMX1A is crucial for the differentiation of these cells. However, LMX1A and LMX1B function cooperatively to regulated the proliferation, specification and differentiation of midbrain dopaminergic neuronal progenitors.
Background References:
  • Hobert O and Westphal H. Functions of LIM-homeobox genes. Trends Genet. 2000; 16:75-83.
  • Nakatani T, Kumai M, Mizuhara E, et al. Lmx1a and Lmx1b cooperate with Foxa2 to coordinate the specification of dopaminergic neurons and control of floor plate cell differentiation in the developing mesencephalon. Dev. Biol. 2010; 339:101-13.
  • Guo C, Qiu HY, Huang Y, et al. Lmx1b is essential for FGF8 and Wnt1 expression in the isthmic organizer during tectum and cerebellum development in mice. Development 2007; 134:317-25.
  • Chizhikov VV and Millen KJ. Control of roof plate formation by LMX1A in the developing spinal cord. Development 2004; 131:2693-705.
Buffer:
LMX1B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal LMX1B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human LMX1B.

The immunogen is located within amino acids 280 - 330 of LMX1B.
NCBI Gene ID #:
4010
NCBI Official Name:
LIM homeobox transcription factor 1, beta
NCBI Official Symbol:
LMX1B
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
NP_001167617
Protein GI Number:
292494913
Purification:
LMX1B Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.
Swissprot #:
O60663
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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