WIPI2 Antibody
| Code | Size | Price |
|---|
| PSI-7103-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7103-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
WIPI2 Antibody: Atg21, ATG18B, CGI-50, WIPI-2, WD repeat domain phosphoinositide-interacting protein 2, WIPI49-like protein 2
Application Note:
WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
WIPI2 Antibody: WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
Background References:
- Smith TF. Diversity of WD-repeat proteins. Subcell. Biochem. 2008; 48:20-30.
- Polson HE, de Lartique J, Rigden DJ, et al. Mammalian ATG18 (WIPI2) localizes to moegasome-anchored phagophores and positively regulates LC3 lipidation. Autophagy 2010; 6:506-22.
- Proikas-Cezanne T and Robenek H. Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J. Cell. Mol. Med. 2011; 15:2007-10.
Buffer:
WIPI2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal WIPI2 antibody was raised against a 17 amino acid peptide near the amino terminus of human WIPI2.
The immunogen is located within the first 50 amino acids of WIPI2.
The immunogen is located within the first 50 amino acids of WIPI2.
NCBI Gene ID #:
26100
NCBI Official Name:
WD repeat domain, phosphoinositide interacting 2
NCBI Official Symbol:
WIPI2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
NP_056425
Protein GI Number:
7661580
Purification:
WIPI2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1.
Swissprot #:
Q9Y4P8
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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