TRIM28 Antibody
| Code | Size | Price |
|---|
| PSI-2521-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-2521-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TRIM28 Antibody: KAP1, TF1B, RNF96, TIF1B, KAP1, Transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, TIF1-beta
Application Note:
TRIM28 antibody can be used for detection of TRIM28 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
TRIM28 Antibody: TRIM28, also known as KAP-1, is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily and contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TRIM28 is a corepressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TRIM28 acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. TRIM28 associates with both heterochromatin and euchromatin, causing gene silencing by both HP1 binding and histone deacetylation.
Background References:
- Friedman JR, Fredericks WJ, Jensen DE, et al. KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev. 1996; 10:2067-78.
- Cammas F, Mark M, Dolle P, et al. Mice lacking the transcriptional corepressor TIF1beta are defective in early postimplantation development. Dev. 2000; 127:2955-63.
- Chang CJ, Chen YL, and Lee SC. Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression. Mol. Cell Biol. 1998; 18:5880-7
- Groner AC, Meylan S, Ciuffi A, et al. KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading. PLoS Genet. 2010; 6:e1000869.
Buffer:
TRIM28 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal TRIM28 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TRIM28.
The immunogen is located within the last 50 amino acids of TRIM28.
The immunogen is located within the last 50 amino acids of TRIM28.
NCBI Gene ID #:
10155
NCBI Official Name:
tripartite motif containing 28
NCBI Official Symbol:
TRIM28
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 92 kDa
Protein Accession #:
NP_005753
Protein GI Number:
5032179
Purification:
TRIM28 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least three isoforms of TRIM28 are known to exist; this antibody will detect all three isoforms
Swissprot #:
Q13263
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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