DHX36 Antibody

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ProSci
Product Code: PSI-7163
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7163-0.02mg0.02mg£150.00
Quantity:
PSI-7163-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of DHX36 in mouse liver tissue lysate with DHX36 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of DHX36 in human liver tissue with DHX36 antibody at 5 μg/mL.
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Western blot analysis of DHX36 in mouse liver tissue lysate with DHX36 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of DHX36 in human liver tissue with DHX36 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
DHX36 Antibody: G4R1, RHAU, DDX36, MLEL1, KIAA1488, Probable ATP-dependent RNA helicase DHX36, DEAH box protein 36
Application Note:
DHX36 antibody can be used for detection of DHX36 by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
DHX36 Antibody: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure. DHX36 (DEAH box protein 36), also known as MLE-like protein 1 and RNA helicase associated with AU-rich element ARE (RHAU), belongs to RNA helicase of the DEAH family and may function in sex development and spermatogenesis. It is expressed in testis and is evolutionary conserved with true orthologs in almost all animal species. DHX36 plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. DHX36 is required for early embryogenesis.
Background References:
  • Cordin O, Banroques J, Tanner NK, et al. The DEAD-box protein family of RNA helicases. Gene 2006; 367:17-37.
  • Linder P. Dead-box proteins: a family affair?active and passive players in RNP-remodeling. Nucleic Acids Res. 2006; 34:4168-80.
  • Lattmann S, Giri B, Vaughn JP, et al. Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. Nucleic Acids Res. 2010; 38:6219-33.
  • Fu JJ, Li LY, Liu SF, et al. Expression research for human DDX36 and mouse Ddx36 gene in the adult testis. Yi Chuan Xue Bao 2003; 30: 201-208.
Buffer:
DHX36 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal DHX36 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DHX36.

The immunogen is located within the last 50 amino acids of DHX36.
NCBI Gene ID #:
170506
NCBI Official Name:
DEAH (Asp-Glu-Ala-His) box polypeptide 36
NCBI Official Symbol:
DHX36
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 111 kDa
Protein Accession #:
NP_065916
Protein GI Number:
167830433
Purification:
DHX36 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least four isoforms of DDX36 are known to exist; this antibody will detect the three longest isoforms.
Swissprot #:
Q9H2U1
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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